Wider , Tanja Schneider and Markus Aebi
نویسندگان
چکیده
Fungi and bacteria compete with an arsenal of secreted molecules for their ecological niche. This repertoire represents a rich and inexhaustible source for antibiotics and fungicides. Antimicrobial peptides are an emerging class of fungal defense molecules that are promising candidates for pharmaceutical applications. Based on a co-cultivation system, we studied the interaction of the coprophilous basidiomycete Coprinopsis cinerea with different bacterial species and identified a novel defensin, copsin. The polypeptide was recombinantly produced in Pichia pastoris and the 3D structure was solved by NMR. The cysteine stabilized α/β-fold with a unique disulfide connectivity and an N-terminal pyroglutamate rendered copsin extremely stable against high temperatures and protease digestion. Copsin was bactericidal against a diversity of Gram positive bacteria, including human pathogens such as Enterococcus faecium and Listeria monocytogenes. Characterization of the antibacterial activity revealed that copsin bound specifically to the peptidoglycan precursor lipid II and therefore interfered with the cell wall biosynthesis. In particular, and unlike lantibiotics and other defensins, the third position of the lipid II pentapeptide is essential for effective copsin binding. The unique structural properties of copsin make it a possible scaffold for new
منابع مشابه
Hemodynamic forces tune the arrest, adhesion and extravasation of circulating tumor cells
Hemodynamic forces tune the arrest, adhesion and extravasation of circulating tumor cells Gautier Follain, Naël Osmani, Sofia Azevedo, Guillaume Allio, Luc Mercier , Matthia A. Karreman, Gergely Solecki, Nina Fekonja, Claudia Hille, Vincent Chabannes, Guillaume Dollé, Thibaut Metivet, Christophe Prudhomme, Bernhard Ruthensteiner, André Kemmling, Susanne Siemonsen, Tanja Schneider, Jens Fiehler,...
متن کاملOpportunities of Telemedicine for Orthopedics and Trauma Surgery
Today telemedicine, or eHealth, is an important tool in everyday medical life. In all areas, from the preclinical to aftercare, significant improvements in communication structures have been seen in various eHealth options, which have had significant, positive effects on the quality of patient care in orthopedics and trauma surgery. Initially, there were several isolated solutions and many smal...
متن کاملBiotin-binding proteins in the defense of mushrooms against predators and parasites.
Tamavidins are fungal biotin-binding proteins (BBPs) displaying antifungal activity against phytopathogens. Here we show high toxicity of tamavidins toward nematodes, insects, and amoebae. As these organisms represent important phyla of fungal predators and parasites, we propose that BBPs are part of the chemical defense system of fungi.
متن کاملAuthor's response to reviews Title: A randomized, double-blind, placebo-controlled clinical trial to evaluate the efficacy and safety of neramexane in patients with moderate to severe subjective tinnitus Authors:
Markus Suckfuell ([email protected]) Michael Althaus ([email protected]) Barbara Ellers-Lenz ([email protected]) Alexander Gebauer ([email protected]) Roman Goertelmeyer ([email protected]) Pawel J. Jastreboff ([email protected]) Hans J. Moebius ([email protected]) Tanja Rosenberg ([email protected]) Hermann Russ ([email protected]) ...
متن کامل